2023-2024学年安徽省七年级学习评价[下学期阶段性练习(一)][各科标题均不同]数学.考卷答案

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)PeckreturnedtotheUSAtoraisemoney.Shereceivedhelpfrommanyscientificorganizations,includingtheMuseumofNaturalHistory.In1908,PeckgatheredthemoneyforanotherexpeditiontoMountHuascaran.Thistime,shehiredtwoSwissguides.Ontheirfirsttrip,oneoftheguidesbecameseriouslyill,andtheentireteamwasforcedtoturnback.Aftertheguiderecovered,theysetoffagain.Theclimbwasextremelydifficult:Theweatherwassocoldthateveryonesufferedfromfrostbite.OneoftheguidestookadvantageofPeck'sdistraction(toclimbthefewremainingfeettothetopsothathecouldsaythathehadgottherebeforeher.AlthoughPeckwasangry,shefocusedonthesuccessinachievinghergoal.OnSeptember2,1908,Annie

(2)为将抗生素抗性基因neo(表达产物可以抵抗抗生素G418)从原有载体中切下以插入到R基因中,据图及下表中限制酶识别位点序列信息,应选择限制酶分别对R基因和ne0基因进行酶切,并用连接,完成打祀载体构建。